JOB OFFER

Position in the project: 

Post-doctoral researcher

Scientific discipline:

Molecular biology and biochemistry

Job type:

Full-time position, employment contract

Number of job offers: 

1

Remuneration/stipend amount/month:

expected net salary approx. 8 500 PLN

Position starts on: 

May, 2021

Maximum  period of contract/stipend agreement:

14 months

Institution:

Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Abrahama 58, 80-307 Gdansk, Poland

Project leader:

Igor Konieczny

Project title:

Condition-dependent protease activation for targeted proteolysis in the regulation of DNA replication

Project is carried out within the Team 5/2017  programme of the Foundation for Polish Science

Project description:

The goal of this project is elucidation how, depending on conditions, proteases are structurally and spatially regulated to degrade essential replication proteins. The research will concentrate on bacterial Lon protease and its phosphate containing cofactors, contributing to the protease activities. By applying in vitro and in vivo experiments, including real-time, single-molecule and single-cell analysis with a variety of Lon proteins, substrates and cofactors, we expect to describe substrate-specific effects on Lon, and relate them to growth conditions. The cutting-edge techniques will be utilized for the description of the structure of Lon complexes. The research on molecular mechanisms of fundamental processes in bacteria is required due to the growing threat arising from the multidrug-resistant bacterial strains. Our goal is to provide data on Lon as a new target for new antimicrobial strategies based on the protease activity control. The project will be conducted with international collaboration of TEAM partners.

Key responsibilities include:

1.performing research using biochemical, genetic and molecular biology techniques (independently and in collaboration with other team members)
2.participation in design of experiments and data analysis
3.participation in preparation and writing of manuscripts
4.supervising younger researchers (PhD students and Master students) involved in the project
5.active participation in lab meetings, departmental seminars and conferences
6.the project may require research stays in TEAM partners laboratories

Profile of candidates/requirements:

1.Doctoral degree in biochemistry or molecular biology
2.Publication record and experimental training in biochemistry and molecular biology. Publications in the field of DNA replication, proteases and chaperones are preferable 
3.Ability to communicate fluently in English (spoken and written)
4.Strong motivation to pursue scientific questions, creativity and analytical thinking
5.Research experience (publication proved) in protein purification, protein-protein interactions, enzymatic activities and genetics.
6.The experience in SPR/BLI, nanoDSF, MST, AFM techniques will be advantageous
7.Candidate should be no longer than 5 years from receiving Ph.D. degree

Required documents:

1.Motivation letter (in English)
2.CV (in English)
3.Two recommendation letters from previous and current supervisors including the PhD project supervisor and the supervisors contact E-mails.

We offer:

-work in active research team in an excellent international  scientific environment
-comprehensive cross-disciplinary training in molecular biology, biochemistry, bacterial genetics and protein structure/function predictions
-participation in scientific seminars and conferences

Please submit the following documents to:

team_konieczny@ug.edu.pl

Application deadline:

April 03st, 2021

For more details about the position please visit:

      https://en.biotech.ug.edu.pl/research/research_groups/laboratory_molecul...

Euraxess job/stipend offer:

https://euraxess.ec.europa.eu/jobs/333870