The LightCycler® 2.0 Instrument allows you to monitor amplification of 32 PCR products simultaneously, in real-time and online, with six different detection channels in multiplex experiments. The LightCycler® Software 4.1 collects fluorescence data from all six channels at each cycle and displays it immediately, enabling you to terminate PCR runs early if signal levels are adequate, or prolong them when signals are too low. Amplification occurs in specially designed reaction capillaries, whose superb surface-to-volume ratio provides extremely rapid thermal transfer, facilitating high-speed thermal cycling. The combination of LightCycler® Capillaries and air-driven temperature control allows an entire 35-cycle run to be performed in as little as 30 minutes (with 20 µl capillaries) to 60 minutes (with 100 µl capillaries).
